sap i cloning site Search Results


ndei sapi sites  (New England Biolabs)
99
New England Biolabs ndei sapi sites
Ndei Sapi Sites, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sap+i+cloning+site/NdeI/10__1074_slash_jbc__m408119200-63-19-26
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sapi restriction sites  (New England Biolabs)
97
New England Biolabs sapi restriction sites
Sapi Restriction Sites, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sap+i+cloning+site/SapI/bio_rxiv__2020__03__25__007740-26-9-16
Average 97 stars, based on 1 article reviews
sapi restriction sites - by Bioz Stars, 2026-09
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sapi sites  (New England Biolabs)
97
New England Biolabs sapi sites
Sapi Sites, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sap+i+cloning+site/SapI/pm24518936-256-17-23
Average 97 stars, based on 1 article reviews
sapi sites - by Bioz Stars, 2026-09
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sgrna cloning site  (Addgene inc)
95
Addgene inc sgrna cloning site
Design of a CRISPRa system for Candida albicans . a) The single-plasmid system contains a catalytically inactive (“dead”) dCas9 protein, paired with the VPR complex of activator domains to cause upregulation at a target locus. The <t>sgRNA</t> region can be subjected to Golden Gate cloning with a custom N20 sequence in order to target the dCas9-VPR complex to a desired region of DNA through complementary base-pairing. The PacI enzyme is used to linearize the plasmid at <t>the</t> <t>NEUT5L</t> locus, which allows the plasmid to be integrated into the cell's chromosome and remain in the cell following cell division. Panel created with BioRender.com. b) Once the sgRNA recognizes and binds to a target sequence of DNA, the dCas9-VPR complex will cause the recruitment of transcription initiation complex factors to the adjacent genetic material, thus increasing gene expression from that region. Panel created with BioRender.com. c) Wild-type C. albicans cells and cells containing a nontargeting CRISPRa plasmid were grown in YPD at 30°C to compare fitness under standard laboratory conditions. Growth was recorded via optical density at 600 nm at 15 min intervals over the course of 24 h. Data points show the mean and standard deviation at each time point ( n = 32).
Sgrna Cloning Site, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sap+i+cloning+site/sgRNA(MS2)+cloning+backbone+(Plasmid+%2361424)/pmc09911074-36-33-15
Average 95 stars, based on 1 article reviews
sgrna cloning site - by Bioz Stars, 2026-09
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sapi bamhi sites  (New England Biolabs)
99
New England Biolabs sapi bamhi sites
Design of a CRISPRa system for Candida albicans . a) The single-plasmid system contains a catalytically inactive (“dead”) dCas9 protein, paired with the VPR complex of activator domains to cause upregulation at a target locus. The <t>sgRNA</t> region can be subjected to Golden Gate cloning with a custom N20 sequence in order to target the dCas9-VPR complex to a desired region of DNA through complementary base-pairing. The PacI enzyme is used to linearize the plasmid at <t>the</t> <t>NEUT5L</t> locus, which allows the plasmid to be integrated into the cell's chromosome and remain in the cell following cell division. Panel created with BioRender.com. b) Once the sgRNA recognizes and binds to a target sequence of DNA, the dCas9-VPR complex will cause the recruitment of transcription initiation complex factors to the adjacent genetic material, thus increasing gene expression from that region. Panel created with BioRender.com. c) Wild-type C. albicans cells and cells containing a nontargeting CRISPRa plasmid were grown in YPD at 30°C to compare fitness under standard laboratory conditions. Growth was recorded via optical density at 600 nm at 15 min intervals over the course of 24 h. Data points show the mean and standard deviation at each time point ( n = 32).
Sapi Bamhi Sites, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sap+i+cloning+site/BamHI/bio_rxiv__576132-182-14-18
Average 99 stars, based on 1 article reviews
sapi bamhi sites - by Bioz Stars, 2026-09
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px330 u6 chimeric bb cbh hspcas9  (Addgene inc)
96
Addgene inc px330 u6 chimeric bb cbh hspcas9
Design of a CRISPRa system for Candida albicans . a) The single-plasmid system contains a catalytically inactive (“dead”) dCas9 protein, paired with the VPR complex of activator domains to cause upregulation at a target locus. The <t>sgRNA</t> region can be subjected to Golden Gate cloning with a custom N20 sequence in order to target the dCas9-VPR complex to a desired region of DNA through complementary base-pairing. The PacI enzyme is used to linearize the plasmid at <t>the</t> <t>NEUT5L</t> locus, which allows the plasmid to be integrated into the cell's chromosome and remain in the cell following cell division. Panel created with BioRender.com. b) Once the sgRNA recognizes and binds to a target sequence of DNA, the dCas9-VPR complex will cause the recruitment of transcription initiation complex factors to the adjacent genetic material, thus increasing gene expression from that region. Panel created with BioRender.com. c) Wild-type C. albicans cells and cells containing a nontargeting CRISPRa plasmid were grown in YPD at 30°C to compare fitness under standard laboratory conditions. Growth was recorded via optical density at 600 nm at 15 min intervals over the course of 24 h. Data points show the mean and standard deviation at each time point ( n = 32).
Px330 U6 Chimeric Bb Cbh Hspcas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sap+i+cloning+site/pX330-U6-Chimeric_BB-CBh-hSpCas9+(Plasmid+%2342230)/pmc08517100-434-32-38
Average 96 stars, based on 1 article reviews
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plasmid ptybii  (New England Biolabs)
99
New England Biolabs plasmid ptybii
Design of a CRISPRa system for Candida albicans . a) The single-plasmid system contains a catalytically inactive (“dead”) dCas9 protein, paired with the VPR complex of activator domains to cause upregulation at a target locus. The <t>sgRNA</t> region can be subjected to Golden Gate cloning with a custom N20 sequence in order to target the dCas9-VPR complex to a desired region of DNA through complementary base-pairing. The PacI enzyme is used to linearize the plasmid at <t>the</t> <t>NEUT5L</t> locus, which allows the plasmid to be integrated into the cell's chromosome and remain in the cell following cell division. Panel created with BioRender.com. b) Once the sgRNA recognizes and binds to a target sequence of DNA, the dCas9-VPR complex will cause the recruitment of transcription initiation complex factors to the adjacent genetic material, thus increasing gene expression from that region. Panel created with BioRender.com. c) Wild-type C. albicans cells and cells containing a nontargeting CRISPRa plasmid were grown in YPD at 30°C to compare fitness under standard laboratory conditions. Growth was recorded via optical density at 600 nm at 15 min intervals over the course of 24 h. Data points show the mean and standard deviation at each time point ( n = 32).
Plasmid Ptybii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sap+i+cloning+site/T4+DNA+Ligase/pm28402361-50-30-46
Average 99 stars, based on 1 article reviews
plasmid ptybii - by Bioz Stars, 2026-09
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sapi ecori site  (New England Biolabs)
99
New England Biolabs sapi ecori site
Design of a CRISPRa system for Candida albicans . a) The single-plasmid system contains a catalytically inactive (“dead”) dCas9 protein, paired with the VPR complex of activator domains to cause upregulation at a target locus. The <t>sgRNA</t> region can be subjected to Golden Gate cloning with a custom N20 sequence in order to target the dCas9-VPR complex to a desired region of DNA through complementary base-pairing. The PacI enzyme is used to linearize the plasmid at <t>the</t> <t>NEUT5L</t> locus, which allows the plasmid to be integrated into the cell's chromosome and remain in the cell following cell division. Panel created with BioRender.com. b) Once the sgRNA recognizes and binds to a target sequence of DNA, the dCas9-VPR complex will cause the recruitment of transcription initiation complex factors to the adjacent genetic material, thus increasing gene expression from that region. Panel created with BioRender.com. c) Wild-type C. albicans cells and cells containing a nontargeting CRISPRa plasmid were grown in YPD at 30°C to compare fitness under standard laboratory conditions. Growth was recorded via optical density at 600 nm at 15 min intervals over the course of 24 h. Data points show the mean and standard deviation at each time point ( n = 32).
Sapi Ecori Site, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sap+i+cloning+site/EcoRI/pm29183694-143-16-22
Average 99 stars, based on 1 article reviews
sapi ecori site - by Bioz Stars, 2026-09
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Design of a CRISPRa system for Candida albicans . a) The single-plasmid system contains a catalytically inactive (“dead”) dCas9 protein, paired with the VPR complex of activator domains to cause upregulation at a target locus. The sgRNA region can be subjected to Golden Gate cloning with a custom N20 sequence in order to target the dCas9-VPR complex to a desired region of DNA through complementary base-pairing. The PacI enzyme is used to linearize the plasmid at the NEUT5L locus, which allows the plasmid to be integrated into the cell's chromosome and remain in the cell following cell division. Panel created with BioRender.com. b) Once the sgRNA recognizes and binds to a target sequence of DNA, the dCas9-VPR complex will cause the recruitment of transcription initiation complex factors to the adjacent genetic material, thus increasing gene expression from that region. Panel created with BioRender.com. c) Wild-type C. albicans cells and cells containing a nontargeting CRISPRa plasmid were grown in YPD at 30°C to compare fitness under standard laboratory conditions. Growth was recorded via optical density at 600 nm at 15 min intervals over the course of 24 h. Data points show the mean and standard deviation at each time point ( n = 32).

Journal: G3: Genes|Genomes|Genetics

Article Title: Development and applications of a CRISPR activation system for facile genetic overexpression in Candida albicans

doi: 10.1093/g3journal/jkac301

Figure Lengend Snippet: Design of a CRISPRa system for Candida albicans . a) The single-plasmid system contains a catalytically inactive (“dead”) dCas9 protein, paired with the VPR complex of activator domains to cause upregulation at a target locus. The sgRNA region can be subjected to Golden Gate cloning with a custom N20 sequence in order to target the dCas9-VPR complex to a desired region of DNA through complementary base-pairing. The PacI enzyme is used to linearize the plasmid at the NEUT5L locus, which allows the plasmid to be integrated into the cell's chromosome and remain in the cell following cell division. Panel created with BioRender.com. b) Once the sgRNA recognizes and binds to a target sequence of DNA, the dCas9-VPR complex will cause the recruitment of transcription initiation complex factors to the adjacent genetic material, thus increasing gene expression from that region. Panel created with BioRender.com. c) Wild-type C. albicans cells and cells containing a nontargeting CRISPRa plasmid were grown in YPD at 30°C to compare fitness under standard laboratory conditions. Growth was recorded via optical density at 600 nm at 15 min intervals over the course of 24 h. Data points show the mean and standard deviation at each time point ( n = 32).

Article Snippet: The plasmid backbone used in this study was the C. albicans -optimized CRISPR-dCas9 plasmid (pRS143, Addgene #122377) used in our previous study ( Wensing et al. 2019 ), containing the NEUT5L integration site, sgRNA cloning site ( SNR52 promoter, SapI cloning locus, and sgRNA tail), and dCAS9 .

Techniques: Plasmid Preparation, Cloning, Sequencing, Gene Expression, Standard Deviation